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Abstract: Spent mushroom substrate (SMS) has been used widely in coal mining regions of the USA as the primary substrate in constructed wetlands for the treatment of coal mine drainage. In laboratory and mesocosm studies, SMS has emerged as one of the substrates for mine water treatment. Provided the pH of the mine water does not fall below 3.0, SMS can be used in the mitigation plan. However, neither Mn nor dissolved ferric Fe appears to be treatable using reducing SMS wetlands. Since after a few years much of the nonrefractive organic carbon in SMS wil have been decomposed and metabolized, carbon supplementation can significantly extend the life of the SMS treatment wetland and improve water treatment. -from Authors
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