Abstract: Euglena mutabilis is an acidophilic, photosynthetic protozoan that forms benthic mats in acid mine drainage (AMD) channels. At the Green Valley mine, western Indiana, E. mutabilis resides in AMD measuring <4.2 pH, with high concentrations of dissolved constituents (up to 22.67 g/l). One of the main factors influencing E. mutabilis distribution is water temperature. The microbe forms thick (>1 mm), extensive mats during spring and fall, when water temperature is between 13 and 28 degrees C. During winter and summer, when temperatures are outside this range, benthic communities have a very patchy distribution and are restricted to areas protected from extreme temperature changes. E. mutabilis also responds to rapid increases in pH, which are associated with rainfall events. During these events pH can increase above 4.0, causing precipitation of Fe and Al oxy-hydroxides that cover the mats. The microbe responds by moving through the precipitates, due to phototaxis, and reestablishing the community at the sediment-water interface within 12 hours. The biological activities of E. mutabilis may have a beneficial effect on AMD systems by removing iron from effluent via oxygenic photosynthesis, and/or by internal sequestration. Photosynthesis by E. mutabilis contributes elevated concentrations of dissolved oxygen (DO), up to 17.25 mg/l in the field and up to 11.83 mg/l in the laboratory, driving oxidation and precipitation of reduced metal species, especially Fe (II), which are dissolved in the effluent. In addition, preliminary electro-microscopic and staining analyses of the reddish intracellular granules in E. mutabilis indicate that the granules contain iron, suggesting that E. mutabilis sequesters iron from AMD. Inductive coupled plasma analysis of iron concentration in AMD with and without E. mutabilis also shows that E. mutabilis accelerates the rate of Fe removal from the media. Whether iron removal is accelerated by internal sequestration of iron and/or by precipitation via oxygenic photosynthesis has yet to be determined. These biological activities may play an important role in the natural remediation of AMD systems.

Abstract: There are basically two kinds of biological passive treatment cells for treating mine drainage. Aerobic Cells, containing cattails and other plants, are typically applicable to coal mine drainage where iron and manganese and mild acidity are problematic. Anaerobic Cells or Sulfate-Reducing Bioreactors are typically applicable to metal mine drainage with high acidity and a wide range of metals. Most passive treatment systems employ one or both of these cell types. The track record of aerobic cells in treating coal mine drainage is impressive, especially in the eastern coalfields. Sulfate-reducing bioreactors have tremendous potential at metal mines and coal mines, but have not seen as wide an application. This paper presents the advantages of sulfate-reducing bioreactors in treating mine drainage, including: the ability to work in cold, high altitude environments, handle high flow rates of mildly affected ARD in moderate acreage footprints, treat low pH acid drainage with a wide range of metals and anions including uranium, selenium, and sulfate, accept acid drainagecontaining dissolved aluminum without clogging with hydroxide sludge, have life-cycle costs on the order of $0.50 per thousand gallons, and be integrated into “semi-passive” systems that might be powered by liquid organic wastes. Sulfate reducing bioreactors might not be applicable in every abandoned mine situation. However a phased design program of laboratory, bench, and pilot scale testing has been shown to increase the likelihood of a successful design.

Abstract: This paper describes how tracer tests can be used in flooded underground mines to evaluate the hydrodynamic conditions or reliability of dams. Mine water tracer tests are conducted in order to evaluate the flow paths of seepage water, connections from the surface to the mine, and to support remediation plans for abandoned and flooded underground mines. There are only a few descriptions of successful tracer tests in the literature, and experience with mine water tracing is limited. Potential tracers are restricted due to the complicated chemical composition or low pH mine waters. A new injection and sampling method ('LydiA'-technique) overcomes some of the problems in mine water tracing. A successful tracer test from the Harz Mountains in Germany with Lycopodium clavatum, microspheres and sodium chloride is described, and the results of 29 mine water tracer tests indicate mean flow velocities of between 0.3 and 1.7 m min^-1.